3D bioprinting is an innovative and rapidly evolving technology that allows for the precise fabrication of living tissues and organs using layer-by-layer deposition of bioinks. This approach combines biomaterials, cells, and growth factors to recreate biological structures with high spatial control. This paper provides a comprehensive overview of the principles, techniques, applications, and challenges associated with 3D bioprinting of human tissues and organs. It highlights the current state of research, suitable bioinks, clinical potential, and future perspectives of this groundbreaking field.